Hi,
I am working on developing several bacterial enzymes for cancer therapy.
They have good therapeutic charateristics like long plasma half life in mice.
We are limited by antibody-mediated inactivation of these enzymes during
repeated treatment cycles. We are trying to shield them from antibody-mediated
inactivation by attaching PEG to the lysine residues. This has increased the
therapeutic life of these enzymes two fold. Also it increases the serum half-
life to 72 hours! Are there better ways of preventing antibody-mediated
inactivation of therapeutic proteins? Are there efficient ways of inducing
tolerance to a specific protein? Any help in this subject would be greatly
appreciated. Thanks.
Natarajan
