Carbodiimides like EDC crosslink -COOH groups to hydarzides or amines. They are zero length
crosslinkers that do not incorporate a cross-bridge when linking. Crosslinking is normally
performed at a pH of 4.5 to 5.0 with a very rapid reaction rate that can be slowed down ( and
made more efficient) by the addition of an NHS ester.The most commonly used reaction buffer is
MES and buffers with phosphates, carboxylic acids, and amines should be avoided.
Tom Brotcke
Technical Assistance
Pierce Chemical Co
Phone: 1-815-968-0747
Fax: 1-815-968-7316
E-mail: PierceChem at mcimail.com
