IUBio

biotinylated antibodies in FACS

David Tak Yan Yu dtyyu at ucla.edu
Wed Sep 6 17:46:02 EST 1995


For looking at cell surface antigens by FACS, would biotinylated antibodies be 
more senstive than unconjugated antibodies.  What is the best way of 
conjugating antibodies with biotin?  What is the best avidin marker?  Is it 
difficult to work out the optimum conc of first and second reagents in 
staining the cells?
David Yu
dtyyu at ucla.edu



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