> Indomethacin adnimistered to Lewis, BN, and F1(Lewis x BN) rats resulted
> in mortality in 4-5 days. Indomethacin was prepared freshly in sodium
> carbonate buffer and administered i.p. or by gavage, 0.5 or 1 mg per
> rat. The recommended dose in the literature is 1 mg/rat/day. I haven't
> come across any report on toxicity at this dosage. It woul be of
> immense help if someone could share his/her experience and suggest
> alternate means to inhibit the cyclooxygenase pathway in rats.
I think you have missed in your dosing. The correct oral dose should be 1
mg/kg/rat. An oral dose of 1 mg in a 250g rat would give you a toxic dose of
4 mg/kg. Rule of thumb is never exceed 3 mg/kg or you'll be guraanteed
toxicity. A dose of 1 mg/kg is standard to maximally block CO activity
without toxicity.
Bob
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