In article <43pkl4$22ak at majestix.uni-muenster.de>, Torsten Boerchers <borcher at uni-muenster.de> says:
>>Dear netters
>>I cannot find the appropriate reference anymore:
>>Does anybody have the conditions for coating
>of microtiterplates (nunc-maxisorb) with
>avidin on hand (buffer, concentration of avidin).
>>Thank you very much for helping me.
>Torsten
>------------------------------------------------------------------------/
> Torsten Boerchers /
> _/ _/_/_/ _/_/_/ Institute of Chemical- and Biochemical Sensor/
> _/ _/ _/ _/ Research (Molecular Biology Group) /
> _/ _/ _/_/_/ Mendelstr 7, D-48149 Muenster, Germany /
> _/ _/ _/ _/ Fax: +49-251-980 2890 Phone: +49-251-980 2876/
>_/ _/_/_/ _/_/_/_/ E-Mail: borcher at uni-muenster.de /
>-----------------------------------------------------------------/
>>Gee - I would just use the Pierce plates - they come either as
8-well strips or as entire plates. They are dry and pre-blocked
when you receive them and they are of very high quality.
Pierce also has NeutrAvidin coated plates (deglycosylated
avidin) - should theoretically have the lowest possible
non-specific interaction.
Keld.