IUBio

coating with avidin

U12201 at uicvm.uic.edu U12201 at uicvm.uic.edu
Thu Sep 21 13:06:50 EST 1995


In article <43pkl4$22ak at majestix.uni-muenster.de>, Torsten Boerchers <borcher at uni-muenster.de> says:
>
>Dear netters
>
>I cannot find the appropriate reference anymore:
>
>Does anybody have the conditions for coating
>of microtiterplates (nunc-maxisorb) with
>avidin on hand (buffer, concentration of avidin).
>
>Thank you very much for helping me.
>Torsten
>------------------------------------------------------------------------/
>                          Torsten Boerchers                            /
>    _/  _/_/_/ _/_/_/    Institute of Chemical- and Biochemical Sensor/
>   _/  _/     _/   _/   Research (Molecular Biology Group)           /
>  _/  _/     _/_/_/    Mendelstr 7, D-48149 Muenster, Germany       /
> _/  _/     _/    _/  Fax: +49-251-980 2890 Phone: +49-251-980 2876/
>_/  _/_/_/ _/_/_/_/  E-Mail: borcher at uni-muenster.de              /
>-----------------------------------------------------------------/
>
>
Gee - I would just use the Pierce plates - they come either as
8-well strips or as entire plates. They are dry and pre-blocked
when you receive them and they are of very high quality.
Pierce also has NeutrAvidin coated plates (deglycosylated
avidin) - should theoretically have the lowest possible
non-specific interaction.
Keld.



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