IUBio

polyclonal antibodies: Help!!

Giorgio Spagnol spagnol at galactica.it
Sun Sep 24 23:20:37 EST 1995


Dear fellow researchers,
I need to raise antibodies to a type 1 membrane glycoprotein of 100 kD.
Since I want these Ab's to act against the extracytoplasmatic peptidic
moyety, and I do not have sufficient quantity of purified antigen, I
tought about using a sinthetic peptide as an immunogen.
Do you have any idea how to choose it, given that the whole sequence of
the protein is known?
Thanks in Advance.
Giorgio.



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