I am just getting starting using an Immuno-Magnetic Separation (IMS) technique for isolation of organisms from
soil and environmental water samples. My purpose is to isolate the organism (crypto, giardia, microsporidia, etc.),
extract the DNA and amplify to determine if the organism is present or not in the test samples. It is known that
common water and soil samples contain inhibitors to PCR, so this technique, in theory, seems like a solution
to this problem. I would like to exchange ideas with anyone with experience using IMS to solve the inhibitor
problem.
Jim Wick
Molecular Biology Resources, Inc.
E-mail: wick at cris.com
Phone: 414-871-7199
FAX: 414-871-1273
Snail Mail: 5520 W. Burleigh St.
Milwaukee, WI 53210
"Don't precipitate more than you can resuspend"
