I need some help, I work making conjugates monoclonal antibodies -
FITC (isotiocyanate fluorescein), but recently several new
fluorescent labeling reagents have been developed that can used in
combination with fluorescein, for example R - phycoerythrin to provide
three color fluorescent. I need some information about of
methodology of conjugates of phycobiliproteins, more exactly
anything of preparation of R-Phycoerythrin - Immunoglobulin (RPE-IgG)
and IgG-RPE-Cy5.
Thanks for advance,
Yamile Perdomo
Center of Molecular Immunology
Havana, Cuba
E - mail>: Yamile at ict.sld.cu
