>We are seeking help with immunizing mice with a DNA plasmid
>construct. Is there anyone with experience in inducing a TH1
>response in mice?
In my previous lab I worked with plasmids to immunise against leishmania
major which requires a Th1 response to cure in mice. As far as I
remember we immunised with around 100ug - 1mg of DNA in sucrose using a
CMV promoter to drive gene expression but I'm not sure if these are
optimal conditions for Th1 responses or not. You might try co-injecting
your plasmid with plasmids encoding IL-12 p40 and/or p35 to push the
response to Th1 but again in our hands this was not neccesary. You
might want to check out a paper in Immunology published in 1995 (I
think) by Xu et al.
Stephen McSorley
