Dear Colleagues:
I am studying the growth inhibition of B cell lymphomas that are
treated with anti-CD40. The CD40 receptor is a growth factor for
normal B cell. It acts by increaseing the expression of Cdk4 and Cdk6,
as well as a survival factor, Bclx(L). We have found that the Cdk4 and
6 are grossly disregulated in lymphomas treated with anti-CD40 mAb;
this is not seen with isotype-matched control antibody. We want to
perform antisense DNA experiments to see if we block the increase in
the Cdks, if the growth inhibition is relieved. A correlate to this
approach would be to make transfectants that have Cdk 4 or 6 under the
control of a an inducible promoter. Therefore, my question is, can any
of you suggest who I should contact for cDNA (human) to the cyclins and
their kinases. I have thought of Drs. Sherr and Reed. Are there
others? Does the ATTCC have these clones?
Please email me at:william.wade at dartmouth.edu
Thank you for your help in this matter.
William F. Wade, Ph.D.
Assistant Professor
Department of Microbiology
