Justin Halls <Justin.Halls at brunel.ac.uk> writes:
>We have a somewhat unusual requirement to perform a fully automated
>mammalian cell viability assessment following thawing from -80C and we
[..]
I am using WST-1 for measurement of PMNs for some time now. I am highly
pleased with it. WST-1 has some distinct advantages over other
Tetrazolium salts like MTT and XTT e.g.:
- there`s no need to solublize WST-1 after it has been cleaved by viable
cells.
- WST-1 is more stable than MTT and XTT and can be stored longer without
degradation
- WST-1 has a wider linear range and shows accelerated color development
It is available through Boehringer Mannheim, Germany. Btw. I`m not linked
in any way with this company.
B. Lorenzen
Pneumological Department, Klinikum Grosshadern, Munich, Germany
