We trying to use immunohistochemistry with two antibodies.
Unfortunately, both of these were raised in mice. Our secondary
antibodies are rabbit anti-mouse. Could someone point me in the
direction of a suitable protocol for labelling with minimal cross-talk?
We have considered the following protocol;
1.Antibody 1
2. Wash
3. Fluroescein conjugated rabbit anti-mouse (at high concentration)
4. Wash
5. Antibody 2
6. Wash
6. Rhodamine conjugated rabbit anti-mouse
Will this protocol work, is there an easier way, or is it a hopeless
task?
Thanks for you help,
Keith Brain
keithb at physiol.su.oz.au
