>Hi, out there...
>>I remember hearing once that in mouse spleen cell proliferation assays, the
>stimulating antigens should not be dissolved in Tris buffers. Can anyone
>elaborate: is this true? Is there something about Tris buffers that is
>incompatible with lymphocyte proliferation assays? If so, can anyone
>explain what it is?
>>Thanks for any advice!
>>Larry
>>Lawrence S. D. Anthony, Ph.D.
>Senior Scientist/Immunology
>StressGen Biotechnologies Corp.
>120-4243 Glanford Avenue,
>Victoria, B.C. V8Z 4B9
>Tel: 604-744-2811
>Fax: 604-744-2877
>Web Page: http://www.stressgen.com/stressgen
I wouldn't touch any cell with a trizma buffered solution. It has a propensity
to buffer the endocytic pathway and no doubt gums up antigen processing in
such assays.
