Scheffer Stephan wrote:
>> Hy, i am looking for some information about
> phagocytic cells, contaminated with
> trinitrotoluene(TNT).
>> Thanks a lot, Stephan Scheffer
> Universitat Marburg ,Germany
>> email: scheffer at papin.hrz.uni-marburg.de
Stephan, perhaps this will help.
--
Jeffrey Smoot
Chemical Abstract Services Phone 614-447-3600 X3198
P.O. Box 3012 E-mail mailto: jsmoot at cas.org
Columbus, OH 43210 WWW http://www.cas.org
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TI Effects of trinitrotoluene (TNT) metabolites on chemiluminescence
response of phagocytic cells
AU Thierfelder, Wulf; Masihi, K. Noel
CS Robert Koch Inst., Berlin, D-13353, Germany
SO Int. J. Immunopharmacol. (1995), 17(5), 453-6
AB The effects of TNT metabolites on the generation of activated oxygen
species was investigated by a sensitive luminol-dependent
chemiluminescence (CL) assay. Spleen cell suspensions contg.
2,4-diaminotoluene, 2,4,6-triaminotoluene, 2-amino-6-nitrotoluene,
4-amino-3,5-dinitrotoluene and 2-amino-4,6-dinitrotoluene were
stimulated with zymosan. Aminotoluenes and amino-nitrotoluenes
induced a dose-dependent inhibition of CL response. The mixed
substituted toluenes generally required higher doses than
aminotoluenes for the suppression of CL response which was not due to
the cytotoxic redn. of cell viability. CL appears to be a
well-suited assay for detn. of the immunotoxic potential of diverse
mols. on phagocytic cells of the immune system.
