We are working on a protocol for intracellular labeling of IL4 and
IFN-gamma in mouse lymphocytes for flow cytometric analysis. Thus far we
stimulated the cells with phytohaemagglutinin (PHA, 50 ug/ml) for 5
hours. Unfortunately, we were not able to detect any cytokine production
using the standard staining protocol of Jung et al. (1993). We do see
blast formation and IL2R expression of our cells.
Does anyone know whether the stimulation protocol we use results in
expression of IL4 and IFN-gamma? If not, what would be a better way to
stimulate our cells?
