Here are two papers where we describe EBV transfection and
cloning under selection. Mouse bone marrow macrophages are also
very good supporters of growth. EBV cells grew best when they
had direct contact with the feeder cells. A feeder monolayer
stayed intact for almost 2 weeks when the feeder cells
themselves where made resistant to e.g. Hygromycin.
If you have more questions please contact me.
Bernhard Maier Ph.D.
Beirne Carter Center for Immunlogy Research
University of Virginia
Charlottesville, VA 22908
Tel. 804 924 1706
Fax. 804 924 1221
E-mail: bm3f at virginia.edu
8. Lucchiari, M., Niedermann, G, Leipner, C., Maier, B.,
Eichmann, K. The mouse bone marrow cell line S17 supports
growth at limiting cel l numbers of EBV-transformed human
lymphoblastoid B cell lines. Immunol. Lett., 1994. 40: p.
189-191.
9. Lucchiari, M., Niedermann, G., Leipner, C., Meyerhans,
A., Eichmann, K., Maier, B., Human immune response To
HIV-1-Nef. I. CD45RO- T lymphocytes of noninfected donors
contain CTL-precursors at high frequency. Int. Immunol., 1994.
6(11).
