The cheapest cytokine assays are bioassays - there are a number
of cell lines which (i) need a particular cytokine or (ii) are
sensitive to a particular cytokine. If you have the time and are
not "rolling in loot" as someone else put it - bioassay may be
the optimal method. Out of interest - how will you know which
cells secreted the cytokines - are you starting with purified
populations?
