In article <4fso3c$hvl at hades.rz.uni-sb.de> Uta Burgard-Wollschlaeger,
utbu0000 at stud.uni-sb.de writes:
>Who is working with flow cytometry to detect intracellular cytokines ?
What are
>your experiences and what do you use: testkit or protocols ?
>Is there anyone working with athletes or on the field of sportsmedicine
with this ?
>Thanks for your help.
>Uta
Pharmingen has a whole series of mouse and human reagents for this
purpose and will send their technique along with it...... It is easy and
quite nice if you are doing multicolour testing ie. surface along with
intracellular. I have used the IFNg human and am quite happy with it...