Dear Group,
We normally prepare thymocyte-conditioned medium (for in vitro
sensitization of B lymphocytes to produce monoclonal antibodies) by
incubating 12 to 14 day old mice thymus in DMEM. This is to produce a
medium containing a mixture of interleukins which are conducive for B
cell growth. I was wondering if anyone who does similar work out there
has looked into adding a recombinant mixture of ILs and other growth
factors as an alternative method to this.
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| Dave Sinasac sinasa2 at server.uwindsor.ca |
| Department of Biological Sciences University of Windsor |
| IRC nickname:Rubisco |
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