IUBio

Efficiently transfectable T- or B-cell lines known ?

Jan Michel Jan.Michel at Klinik.Uni-Regensburg.de
Wed Jul 3 22:35:17 EST 1996


Hello,
I have problems with transfecting Jurkat or Raji cells by electroporation 
with efficiencies higher than about 7 %. Does anybody know a human T- or 
B-cell line that can be better transfected ?
I'm using the Biorad pulser at 200 V / 960 µF.

Thanks
                     Jan



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