In article <4s3aog$g8m at unix13.u-3mrs.fr> Serge Birman,
birman at lnf.cnrs-mrs.fr writes:
>I just purchased a monoclonal antibody. This Mab is shipped lyophilized
>and the company recommends to dissolve it in water and keep in the
>fridge. However, for longer storage, I would like to store it in
>aliquots in the freezer. Is that possible without losing too much
>activity? Which buffer should I use? With or without glycerol? I was
>wondering whether glycerol can be harmful to some antibody.
this question is raised periodically on this board. mAbs are very stable
in PBS at 4C if stored at 1mg/ml or in a carrier solution of BSA or
glycerol. We store both conjugated and unconjugated Abs for years under
these conditions. Freezing is actually harmful esp. for conjugated
Abs.....
