In article <Brian_Freeman-1007961319050001 at mcdbg29.med.nyu.edu> Brian_Freeman at brown.edu (Brian Freeman) writes:
>Hello,
>I am looking for help in isolating Neutrophils from rat/mouse serum in
>sufficient quantity.
>Please post or email freemb01 at popmail.med.nyu.edu.
>Thank you!
>Brian Freeman
>NYU Medical Center
The best method (in my experience) for separation of PMNs from human blood is
lysis of RBC in isotonic NH4Cl followed by differential centrifugation,
described in :Eggleton et al, J.Immunol.Meth 121, 1989, 105-113
It gives much higher yield than using separating media, which could be
important with small volumes of blood.
BTW, it has to be fresh blood, not serum.
Hope it helps,
Eva
