Brian Freeman (Brian_Freeman at brown.edu) wrote:
: Hello,
: I am looking for help in isolating Neutrophils from rat/mouse serum in
: sufficient quantity.
There is a product called Polymorphprep available. It is made by Nycomed
Pharma AS. They are Norwegian but there is probably a US supplier. It is
a sort of high density Ficoll paque: you spin blood over it, like a Ficoll
separation, but you get two interfaces instead of one, and the lower one
contains the neutrophils. I have used it for separating human neutrophils
and it works very well. The original reference is Ferrante & Thong, 1980,
J. Immunol. Methods vol. 36, p. 109.
: Please post or email freemb01 at popmail.med.nyu.edu.
: Thank you!
: Brian Freeman
: NYU Medical Cente
Andrew Fell
QIMR
Brisbane