IUBio

Seek CD45 Cell Based Assay

CBogowitz cbogowitz at aol.com
Sun Jul 14 19:07:45 EST 1996


I am in the process of discovering small molecule inhibitors of CD45.  I
have a great primary screen and have found lead compounds.  I now need to
design a secondary cell based assay.  Literature describes the use of the
human T cell  line Jurkat.  I found that these cells are proliferating
even without stimuli (specific or non-specific).  I'm wondering if I
should use a mixed lymphocyte population (whole blood), but am not sure
how I could determine a CD45 specific event.  Can anyone lend me their
experience in this area?

Truly greatful in advance,

Cheri Bogowitz
CBogowitz at aol.com



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