I am in the process of discovering small molecule inhibitors of CD45. I
have a great primary screen and have found lead compounds. I now need to
design a secondary cell based assay. Literature describes the use of the
human T cell line Jurkat. I found that these cells are proliferating
even without stimuli (specific or non-specific). I'm wondering if I
should use a mixed lymphocyte population (whole blood), but am not sure
how I could determine a CD45 specific event. Can anyone lend me their
experience in this area?
Truly greatful in advance,
Cheri Bogowitz
CBogowitz at aol.com