You seem to be confusing the issues:
DEAE is an ion-exchanger, i.e. NOT affinity in the sense of
a specific purification method.
However DEAE has been used for purification of PC Abs.,
but the outcome is NOT affinity purified Ab.
If you want to specifically purify the antibodies against
your antigen, by far the most common method is to
immobilize the antigen on any number of supports (usually
they are called "crosslinked agarose" or "Sepharose" -
the latter being Pharmacias tradename for crosslinked agarose).
Depending on the functionalities of the antigen (i.e. do you
have NH2 groups, SH groups, or others?) you select a
support that will bind through that functionality.
A good resource is the Harlow and Lane book on antibodies
- it is a Cold Spring Harbor publication - check your library -
it has good protocols etc.
But to sum it up: affinity purification requires the immobilization
of the antigen. Other methods, such as DEAE or Thiophilic adsorption
or even protein A or protein G columns will purify ALL the antibody
in the sample, i.e. the product is not an "affinity purified" Ab.
Keld
