Does anyone have any advice on purification of rat IgM.
The methods I know about are:
(a) Gel filtration
(b) Dialysis against water to precipitate IgM
(c) boric acid precipitation
(d) purification on Protein G - obviously only pulls out monomer.
(e) affinity purification on anti-rat IgM column
Which, if any of these, is the best method?
Similarly, is it feasible to make affinity columns with low affinity IgM?
Thanks in advance
Cath Derry
