IUBio

TRITC conjugated mAb

Keld Sorensen KeldS at uic.edu
Mon Jul 22 13:05:15 EST 1996


I don't have a handy source for fluorochrome labeled Abs, 
however, labeling an antibody with (say) an NHS-dye 
should be entirely equivalent to labeling with NHS-Biotin,
i.e. very easy and quick. 

I did publish a paper on labeling with NHS-biotin 
within a slide-a-lyzer (from Pierce) - the same
method can be used for dialysis tubing or you
could use the "old" method of doing the reaction
in a test tube, followed by desalting or dialysis.

Most researchers don't realize that an antibody
can be labeled in a few hours with 15 min of 
hands-on-time.

Keld.



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