I can't supply references, but this is possible. In some cases the material
cannot be recovered, but in many cases you may sonicate this material and
recover activity.
If you are diluting before freezing, assure that the dilutent contains a
protein source (e.g., BSA).
PJLev bMxV <pjlevbmxv at aol.com> wrote in article
<4sotjr$ane at newsbf02.news.aol.com>...
> We have observed loss of detection of IgM in sera which have undergone
> multiple freeze/thaw cycles. Is there literature which supports and
> explains this observation?
>