IUBio

murine B-cell columns

Cherry Singh singhc at a1.lldmpc.umc.dupont.com
Tue Jul 23 10:41:35 EST 1996


Yes a company by the name of Applied Immune Sciences, Menlo Park, CA.
phone: 415-326-7302 supplies MicroCELLector Mouse anti Rat T-25 cell 
culture flasks for the selection of rat monoclonal antibody labeled 
cells. I have used these flasks for the selection of murine B cells by  
eliminating the T cells using an antibody cocktail consisting of 
anti-Thy and anti-CD3. They are good in enriching for B cells though 
they do not get completely rid of the T cells, I have found that using a 
cocktail of antibodies works better and also do not load a large number 
of labeled cells on the flasks. The advantage is that they are much 
cheaper than using the Dynabeads and you also do not have to get the 
antibody off the cells since it works by negative selection. 





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