IUBio

Dynabeads

Dave Peritt peritt at mail.med.upenn.edu
Thu Jul 25 07:10:07 EST 1996


In article <4t4a8h$eh6 at cc-server9.massey.ac.nz> Richard Johnson,
R.D.Johnson at massey.ac.nz writes:
>Has anyone had any experience (positive or negative) with using antibody
>coated dynabeads to purify proteins from crude bacterial lysates. Despite
>the claim by dynal that this is possible, we have had very limited
>success with extremely low recoveries. 

What is the purpose of using the expensive dynabeads for protein
purification via Ab when you could use a well seasoned and tried
sepharose or other cheap matrix bound Ab??



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