IUBio

ScFv-antibody expression???

Danny T M Leung b574713 at mailserv.cuhk.hk
Wed Jul 31 08:59:23 EST 1996


Dear phage-library workers'
	I would like to know whether ScFv-antibody normally expressed in pIII protein can be 
expressed in pVIII protein. If not, are there any ways to increase ScFv-antibody copy 
number per phage which is about 1-5 copies per phage in the case of pIII protein? The 
problem I found is the ScFv-Ab phage derived from my hybridoma  binds only weakly to 
the antigen.



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