Danny T M Leung (b574713 at mailserv.cuhk.hk) wrote:
: Dear phage-displayed Ab workers,
: I have been working on ScFv antibody expressed on pIII coat protein.
<snip>
: Does anybody has the exprience of expressing ScFv-antibody on pVIII protein?
: Is it too large for expression? If so, are there any alternatives to
: increase the copy number of ScFv per phage?
Hi Danny,
There are some informations that you need to include in the question,
especially what construct you have been using for display; is it a phage
or a phagemid vector?
If the latter is the case, you will probably have monovalent display, i.e.
one displayed scFv per phage particle. If so, the number can be increased
to 3-5 copies per phage by switching to a phage system which will result
in display on all pIII on the phage.
If you are using a phage vector already, pVIII COULD be a solution, but
this would hve to be in a phagemid vector so that wild type pVIII is
present. I cant say if it is possible though... Maybe somebody else
knows of the size limitations for pVIII fusion? Peptides hve been displayed,
but 27 kDa is another matter.
Good luck,
Troels Wind
Denmark
