In article <4m7ssn$5vg at mserv1.dl.ac.uk> Neilx., mabbott at bbsrc.ac.uk
writes:
>I have a question,
>>In an MLR reaction, will responding lymphocytes, from mutant knock-out
mice
>lacking a
>particular cell surface molecule, proliferate in response to stimulation
>by the corresponding wild-type cells?
The MLR reaction is mainly an allogeneic response. Therefore your two
cell types will not have much proliferation since they are from the same
species. I think your question is will the KO mouse recognize and
proliferate to the surface marker since it is absent. This is a very
minor Ag. Therefore you may get some reaction but it will not be
measurable via 3H thymidine since the frequency of specific T cells
against the Ag is very low. allo response has a much higher frequency.
If you let the culture go for a long time with IL-2 you may get eventual
outgrowth of a specific line...But this may not be what you need...
