I am having troubles detecting an antigen using FITC labelled secondary
antibodies. The antigen is in very low amounts and in a tissue with hight
autofluoresence. To make the green label more visible I stained the tissue
with EVANs blue. This technique gives a red color to the negative tissue and
improves visualization of the green fluorescence to the naked eye. However,
when taking pictures of the site, the opening of the lens is retarded by the
red fluorescence and does not pick up the green fluorescence. I have tried
using different filters but cannot get pictures that I can use for
publications. Can anyone recommend a better approach!
Thanks,
Aida Cancel
axc19 at psu.edu
