In article <4m7ssn$5vg at mserv1.dl.ac.uk>, "Neilx." <mabbott at bbsrc.ac.uk> wrote:
> Hi Netters,
>> I have a question,
>> In an MLR reaction, will responding lymphocytes, from mutant knock-out mice
> lacking a
> particular cell surface molecule, proliferate in response to stimulation
> by the corresponding wild-type cells?
>> (Both cell populations bear the same H-2 type).
>> Many thanx in advance
>> Neil Mabbott
> Edinburgh
>> email neil.mabbott at bbsrc.ac.uk
If they are H-2, Mls etc identical it would presumeably need the
equivalent of a minor H difference to get any response at all. Would
depend on what your KO'ed molecule is.
Presuming here that some peptides from your knocked out molecule find
their way into H-2 molecules?
And you don't expect to see minor H in a conventional MLR (anti-class II
and mainly on DC's) - seems unlikely but there's probably literature on
it. Maybe need a second round of stimulation, cytotoxicity rather than
prolif (as per H-Y). Have you tried skin grafting?
--
John
