In article <l.mclean-0305961648300001 at l.mclean.mmm.auckland.ac.nz>,
l.mclean at auckland.ac.nz (Lachy McLean) wrote:
> In article <4m7ssn$5vg at mserv1.dl.ac.uk>, "Neilx." <mabbott at bbsrc.ac.uk> wrote:
>> > Hi Netters,
> >
> > I have a question,
> >
> > In an MLR reaction, will responding lymphocytes, from mutant knock-out mice
> > lacking a
> > particular cell surface molecule, proliferate in response to stimulation
> > by the corresponding wild-type cells?
> >
> > (Both cell populations bear the same H-2 type).
> >
> > Many thanx in advance
> >
> > Neil Mabbott
> > Edinburgh
> >
Well, when cells from H-2 identical mice with different TCR were mixed
(which seems to match the situation you describe) we didn't get a
significant MLR. As some other people have commented, you are probably
looking at a very minor antigen here, so it is unlikely to drive a
reaction as strong as an MLR.
Cheers, Mark
