FITC labelled fluorescence

[Jean-Philip Truman]

In article <4mgt51$o7g at piglet.cc.uic.edu>, rlamot1 at icarus.uic.edu 
says...
>
>In article <axc19.3.3187E17D at psu.edu> Aida Cancel, axc19 at psu.edu 
writes:
>>I am having troubles detecting an antigen using FITC labelled second
>
>Try using PE-labeled antibodies. I haven't had an experience in tussue
>staining, but I've noticed that cells usually have much less
>autofluoresence in red spectra and using PE-labeled antibodies with
>flow-cytometry I was able to detect cells expressing low levels of an
>antigen, while FITC couldn't provide such sensitivity. So it's likely
>that PE-antibodies could work better than FITC-Ab in tussiue staining 
as
>well.
>
>Leonid

Be careful when using PE-conjugated Abs under fluorescent microscopy. 
I've found that while it's good for FACS, it is very quickly 
photobleached and is extinguished before I could get any good pictures.