IUBio

Membrane-impermeable Reducing Agents

brett brett at BORCIM.WUSTL.EDU
Wed May 8 16:00:09 EST 1996


>I am looking to reduce disulphide bonds outside of cells without 
>affecting internal disulphides.  Is there a reducing agent I can use 
>which is membrane impermeable.  Alternatively is there an oxidising 
>agent that is membrane impermeable?
> 
>Thanks
>
>Mike Daws

Look up MESNA (mercaptoethamsulfonic acid, i think), or use glutathione


Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain




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