>I am looking to reduce disulphide bonds outside of cells without
>affecting internal disulphides. Is there a reducing agent I can use
>which is membrane impermeable. Alternatively is there an oxidising
>agent that is membrane impermeable?
>>Thanks
>>Mike Daws
Look up MESNA (mercaptoethamsulfonic acid, i think), or use glutathione
Brett Lindenbach
Program in Immunology
Washington University - St Louis
brett at borcim.wustl.edu
"I own my own pet virus. I get to pet and name her." - Cobain