Michael Daws (Michael.Daws at ucmp.umu.se) wrote:
> I am looking to reduce disulphide bonds outside of cells without
> affecting internal disulphides. Is there a reducing agent I can use
> which is membrane impermeable. Alternatively is there an oxidising
> agent that is membrane impermeable?
>> Thanks
> Mike Daws
How about glutathione? I don't knoe how much to use, but this is the
reducing agent used cytoplasmically to prevent disulfide bonds in cells.
Mike Corboy
University of Texas at Dallas
