In article <bilha001-1205961553460001 at dialup-1-22.gw.umn.edu>,
bilha001 at gold.tc.umn.edu (Julaine) wrote:
> I plan to do immunohistochemistry looking at a nuclear antigen on two
> color FACS sorted cells and cytospun on to a slide. My primary antibody
> for the nuclear antigen is of the same mouse isotype as the primary
> antibody used to sort with the FACS. I predict a cross reaction problem
> when I come back with the secondary Rabbit anti-mouse labled antibody. Any
> quick advice on how to eliminate this background will be appreciated.
> A reply by email is fine bilha001 at gold.tc.umn.edu
protease treatment?
michelle
--
db
or Michelle
