Sorry all,
some-one has been messing with the settings on my reader - the originator of this reply
was actually me.
P.Kwasowski at surrey.ac.uk
Pete
> >
> > From: surglab1 at is.rvh.mcgill.ca (surgical residence)
> >
> > Subject: Protein A columns-Which one should I use?
> >
> > Date: Mon, 13 May 1996 21:23:00 GMT
> >
> > Hello,
> >
> > I'm looking for an protein A- affinity column to purify a mouse
> IgG2b
> > monoclonal antibody from a hybridoma. Does anyone have a protocol
> and
> > can recommend what product is best?
> >
> > (We don't have any chromatographic equipment)
> >
> > JLP
> > surglab1 at is.rvh.mcgill.ca> >
> ----------------------------------------------------------------------
>> Why don't you try the material made by BioProcessing Corp. It's called
> Prosep-A. The
> protein A is immobilized on porous glass, this makes it very robust,
> and usefully it
> settles out of suspension very quickly. So if pushed you could use it
> in a bottle, on a
> roller, with a pasteur-pipette and a steady hand.
>> Hope this helps,
>> Pete
