>> From: surglab1 at is.rvh.mcgill.ca (surgical residence)
>> Subject: Protein A columns-Which one should I use?
>> Date: Mon, 13 May 1996 21:23:00 GMT
>> Hello,
>> I'm looking for an protein A- affinity column to purify a mouse IgG2b
> monoclonal antibody from a hybridoma. Does anyone have a protocol and
> can recommend what product is best?
>> (We don't have any chromatographic equipment)
>> JLP
>surglab1 at is.rvh.mcgill.ca> ----------------------------------------------------------------------
Why don't you try the material made by BioProcessing Corp. It's called Prosep-A. The
protein A is immobilized on porous glass, this makes it very robust, and usefully it
settles out of suspension very quickly. So if pushed you could use it in a bottle, on a
roller, with a pasteur-pipette and a steady hand.
Hope this helps,
Pete
