In Article <4nf6j5 at news-ipg.umds.ac.uk>, erol <b.carey at umds.ac.uk> wrote:
>Anyone aware of the current thinking on the numbers of V,D and J segments in
T and B cells?
>Thanks very much.
>>
well, i think its mostly known. hood's group has sequenced the entire TCR
alpha/delta locus in human and mouse, so you can look that up. i am not up
to date on alpha/beta's. i am pretty sure that there are 125 VH genes
distributed in 2 places/loci (human), although only 51 of them are
functional (these functional ones are on 1 chromasome, and depend on your
genetic background). There are 30 DH's and 6JH's followed by the gambit of
CH's. this is reviewed in a recent imm today. the lambda and kappas are
also pretty well known, i think that there are 50-100 lambdas w/ 7 or so
clusters of some functional, some pseudo genes in a j-C organisation (like
shark clusters). The kappas have bout 85 VK 1-5 JK and 1 C. i think that
lambdas have pseudogenes all over other chromosomes, but am not really sure.
it seems that the only true translocon arrangement seems to be VH's and VK.
ralph
ps, if you need some refs, email me.
R.M. Bernstein PhD
Dept of Micro/Immuno
University of Arizona
Ph: 520 626 2585
Fx: 520 626 2100
url: http://lamprey.medmicro.arizona.eduhttp://radon.gas.uug.arizona.edu/~bernster
