IUBio

HLA typing of MHC class1

Jonathan Kurtis Jonathan_Kurtis at Brown.edu
Thu May 16 16:07:42 EST 1996


Hi all,

I will be doing some HLA class 1 typing of about 100 individuals from
Western Kenya. I will be using cryopreserved PBMCs as source material.
Does anyone have any thoughts as to which method is easier/better (PCR vs.
Ab+Complement). Are there other techniques?? Any advice on companies that
sell kits or usefull references??  I am particularly interested in the B
locus.

Thanks for your help,

jake

-- 
Jonathan Kurtis, Ph.D.
International Health Institute
Brown University Box G-8033
Providence, RI  02912  USA
Jonathan_Kurtis at Brown.edu



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