HLA typing of MHC class1

[Tissue Typing Dept]

For class I work I personally think that serology is the easiest and
cheapest. 

Derek O'Neill 
Tissue Typing Dept.
Beaumont Hospital 
Dublin 

tt at iol.ie


Jonathan_Kurtis at Brown.edu (Jonathan Kurtis) wrote:

>Hi all,

>I will be doing some HLA class 1 typing of about 100 individuals from
>Western Kenya. I will be using cryopreserved PBMCs as source material.
>Does anyone have any thoughts as to which method is easier/better (PCR vs.
>Ab+Complement). Are there other techniques?? Any advice on companies that
>sell kits or usefull references??  I am particularly interested in the B
>locus.

>Thanks for your help,

>jake

>-- 
>Jonathan Kurtis, Ph.D.
>International Health Institute
>Brown University Box G-8033
>Providence, RI  02912  USA
>Jonathan_Kurtis at Brown.edu








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