IUBio

Phycoerythrin stabilisation for microscopy?

Chris Barry chbarry at llnl.gov
Tue May 21 14:20:47 EST 1996


Julian F Dye wrote:
> 
> Hi
> 
Have you tried to put filters in front of the excitatory light source to
decrease the intensity. Also, Are you doing a single antibody staining?
If not, perhaps you might want to tag the antigen twice. ie:

antigen---antibody (rabit) phycoerythrin---anti-rabit IgG phycoerythrin

or something like that.



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