Masaru Kimura (kimuchan at aichi-med-u.ac.jp) wrote:
: I am trying to demonstrate mapping of brain type nitric oxide synthase
: (NOS) in the rat brain using alcohol fixed paraffin sections with
: policlonal rabbit antibody against the amino acid sequence in rat's brain
: type NOS which is different from other type of NOSs. Most of neurons in
: the sections pretreated with trypsin were stained by not only specific
: antibodies but also serum components (perhaps IgG) from nonimmun aniamls
: which were the same kind of animals used to obtain the first antibody
Could you please let me know why should you pretreat with trypsin even when
the antibody is known to recognise aminoacid sequence of the protein.
Raju
.
