There was a method developed in the 70's by the group led by
Avrameas (who developed enzyme immuno-conjugates). Search under
Uriel. It uses a bed of acrylamide-agarose beads (Ultrogel). I
would uses AcA 34 for your MW protein. You place the beads in an
electrophoresis cuvette. Not to much buffer so that you have a
slurry. Dig a trench with a spatula and load your sample. Apply
current and when finished, you can either (1) Use a ruler to
guide a spatula and take cross sections of the gel or (2) get a
grid made that you sink onto the gel and that isolates say 1 cm
transverse sections which you then scoope out. I've used it and
it works fine. You can re-use the gel and re-separate your
fractions if still contamined. You can load a lot of protein. You
could use any other gel too (Sephacryl, etc). Good luck.
Should you wish to reply do so by e-mail since I don't come in
very often here. Ricardo
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Ricardo Moro
