In article <31A6F345.35A8 at sydney.edu.au> Kathrine Wilson <wilson at sydney.edu.au> writes:
>Hi its me again. Growing some spores for my research. Growing in
>cooked meat medium mixed with blood agar at 37C at anerobic conditions
>on standard agar plates. Unfortunately I am having trouble
>distinguishing the toxin produced. I am using pure spores and have
>developed both white dots and brown/black ones. I anyone knows what
>colour they should be, it would help.
>Kathrine
>wilson at sydney.edu.au
This is really starting to get "scary". From the description of your post,
you present to me that you really don't yet know what you are doing, yet
you are "playing around with Cl. botulinum and trying to make bot toxin!!"
I'm sorry if this is strongly worded but I would not want to be on the lab
bench next to someone playing around with Cl. botulinum unless they knew
exactly what they were doing. I would recommend that you please collaborate
with someone in your institution who has an appreciation for the dangers
involved and can help you get guidance on what you are doing. For instance,
have you contacted (by phone, Email, FAX, whatever) someone else in the field
who actually does research with Cl. botulinum (obtained from research
literature) on these aspects? Are you yet immunized against bot toxin? Have
you checked with safety officers at your institution to make sure you have
followed expected procedures in working with such toxic agents? Although
the internet is a wonderful tool in obtaining informative help, why rely on
responses from the internet which may/may not be accurate or safe. I hope you
take these comments constructively as I (and other colleagues who have
responded to your original post) only have your safety in mind.
Regards, Peter Muriana
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Peter M. Muriana
Assistant Professor 317-494-8284 TEL
Dept. of Food Science 317-494-7953 FAX
Purdue University murianap at foodsci.purdue.edu
Smith Hall http://www.foodsci.purdue.edu/
W. Lafayette, IN 47907-1160 personnel/muriana.html
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The opinions expressed above are mine alone and do not represent
endorsement by my employer
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