Chicken IgG from Egg White, Info please?

[Larry Winger]

Many thanks to those respondents who suggested that egg yolk was a better
target, and contributed references -- stimulated me to get off me
backside, trot up to the library, and check out Medline for meself!

There found two contemporary references with easy access in JIM:

Akita, E.M. and Nakai, S.  (1993)  Comparison of four purification methods
for the production of imunoglobulins from eggs laid by hens immunized with
an enterotoxigenic E. coli strain.  J. Immunol. Methods  160:207-214

McLaren, R.D., Prosser, C.G., Grieve, R.C.J., Borissenko, M. (1994)  The
use of caprylic acid for the extraction of the immunoglobulin fraction
from egg yolk of chickens immunised with ovine alpha-lactalbumin.  J. 
Immunol. Methods 177:175-184


Of the two, the caprylic acid method looks the easiest and most
straightforward, to my way of thinking, especially for secondary school
students doing A level extended practicals.

Thanks again, traa . . .

Larry

Larry 'wearing three hats in these cold times' Winger

Larry.Winger at ncl.ac.uk     http://georgia.ncl.ac.uk
LarryWinger at mkn.co.uk      http://mkn.co.uk/help/extra/craftnet/info
law3 at student.open.ac.uk    PGCE Secondary Science, Teacher Trainee,OU